|
|
|
|
이미화 ( ) - 진주보건대학 임상병리과
|
|
Abstract
|
|
|
|
|
Many prospective studies have now confinned the predictive value of plasma fibrinogen levels. Several polyinorphisms of the /3 fibrinogen gene have been investigated in relation to plasma fibrinogen levels.
But, studies of the possible associations between these polymorphisms and plasma fibrinogen concentrations iii the Korean population have not been carried out.
For this study, the blood samples for DNA were collected from 109 healthy Koreans who have no relationship by blood(67 males and 42 females) in due consideration of some other factors such as gender, age, and smoking status. Four polymorphisms of the 6 fibrinogen gene that consist of HaeffI, Alul, JWnll, and BcII restriction fragment length polymorphisms (RFLPs) were investigated to examine the associations between RFLPs and plasma fibrinogen levels. The investigation into the RFLPs were made by polymerase chain reaction and enzyme digestion. The alleles with the restriction site and the non-cleavable alleles wesssre designated Hi and 1-12 for the Haelll polymorphism, Mi and M2 for Mn/I polymorphism, Ai and A2 for the Alul polymorphism, and B2 and Bi for the BcII polymorphism, respectively.
The results were as follows:
1. Genotype HIHi and HIH,_ in HaeIII RFLPs were detected from 69(61.5%) and 40(38.5%) persons among the above 109 control subjects respectively. Genotype MiMi and MiM2 in IVInIl RFLPs from 70(64.2%) and 39(35.8%) persons respectively. Genotype ALAI and AlA2 in Alul RFLPs from 69(61.5%) and 40(36.7%) persons respectively.
Among 95 control subjects, genotype BiBi and BiB2 in BcII RFLPs were shown in 70(73.7%) and 25(263%) persons respectively. The HaeIII, AIuI, lhlnll RFLPs were in tight linkage disequilibrium, while BcII RFLPs not being in it.
2. The significant associations between. Haelll, Alul, MnII RFLPs(HiH2, MIM2, AIAD and plasma fibrinogen were ascertained in both aged people(->_ 50 years) and smokers, whereas the assoc"!. tions between tic1l RFI-Ps and plasma fibrinogen wE-re absent showing no connection with age or smoking status.
3. Plasma fibrinogen concenirati~rns were significantly higher in the old ages(__>_ 50 years) than in the younger ages(_<- 49 years) in male, and also higher in smokers than in nonsmokers.
In conclusion, genetic polynalorphisms(HnH-a, M1,1\42, AiA2) of the S fibrinogen gene are associated and increased with plasma fibrinogen levels. These findings suggest that variation at the fibrinogen gene locus may contribute to the differences in plasma fibrinogen level.
|
|
|
KeyWords
|
|
|
|
|
원문 및 링크아웃 정보
|
|
|
|
|
|
등재저널 정보
|
|
|
|
|
|
|
|